Purification of casein kinase I and isolation of cDNAs encoding multiple casein kinase I-like enzymes.
نویسندگان
چکیده
منابع مشابه
Purification and characterization of casein kinase I from broccoli.
Casein kinase I from broccoli was purified approximately 65,000-fold by chromatography on phosphocellulose, phenyl-Sepharose, CM-Sephacel, and affinity chromatography on N-(2-aminoethyl)-5-chloroisoquinolone-8-sulphonamide (CKI-7)-Sepharose. The catalytic subunit of casein kinase I was identified as a 36-38 kDa polypeptide doublet by using the technique of activity gel assay after SDS/PAGE with...
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In the present study, the molecular cloning and characterization of a 49-kDa form of casein kinase (CK)I from Dictyostelium discoideum is reported. The predicted amino acid sequence shares 70% identity with the catalytic domain of the mammalian delta and epsilon isoforms, Drosophila CKIepsilon and Schizosaccharomyces pombe Hhp1, and 63% identity with Hrr25, a 57-kDa form of yeast CK involved in...
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The Wnt/ -catenin signaling pathway is important in both development and cancer. Casein kinase I (CKI ) is a positive regulator of the canonical Wnt pathway. CKI itself can be regulated in vitro by inhibitory autophosphorylation, and recent data suggest that in vivo kinase activity can be regulated by extracellular stimuli. We show here that the phosphorylation state and kinase activity of CKI ...
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Alzheimer’s disease (AD) is associated with accumulation of the neurotoxic peptide amyloid(A ), which is produced by sequential cleavage of amyloid precursor protein (APP) by the aspartyl protease -secretase and the presenilin-dependent protease -secretase. An increase of casein kinase 1 (CK1) expression has been described in the human AD brain. We show, by using in silico analysis, that APP, -...
متن کاملInvolvement of casein kinase I in cytokine-induced granulocytic differentiation
Two closely related casein kinase I (CKI) isoforms, CKI and CKI , are ubiquitously expressed in many human tissues, but their specific biologic function remains to be clarified. Here, we provide the first evidence that CKI is involved in hematopoietic cell differentiation. CKI , but not CKI , was down-regulated along with human granulocytic differentiation. The specific down-regulation was obse...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1991
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.88.21.9548